Whole Genome Resequencing

NGB diagnostics offers multiple tiers of Resequencing portfolio to help you achieve your Phylogenomics sequencing project goals for a reasonable price. Whether you are starting with raw material or need help analyzing data produced elsewhere, let our team of experienced scientists help.

We currently offer multiple sequencing platforms including Illumina HiSEQ4000 & X10. We can generate low to high deoth genome reequencing for various applications such as:

  1. Population Structure analysis.
  2. Bulk seggregation analysis.
  3. Whole genome Population genomics : Selective sweeps, IBD, IBZ, LD analysis etc.
  4. QTL analysis & fine mapping.

Standard Bioinformatics includes:

  • Mapping on reference genome.
  • SNP and In-Del calling
  • An optional imputation of the variants can be done to impute missing data.
  • Genome Annotation & Comparative Genomics
  • Phylogenomics analysis for sequenced land races/ wild populations to identify selective sweeps.
  • IBD analysis & IBH analysis.
  • LD analysis.
  • Population structure analysis.
  • PHASING and HAPLOTYPE analysis
  • Phylogenetics using Maximum likelihood / Maximum parsimony methods (eg algorithms PhyML, RAxML, NJtree, MEGA etc).
  • QTL analysis: GLM & MLM method. Manhattan Plots. QQ plots.
Whole Genome Resequencing