SAMPLE REQUIREMENTS
Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, NGB Diagnostics has extensive sample quality control procedures to ensure submitted samples conform to requirements for downstream processing.To guarantee the normal processing of your project, samples should meet the standards given below
Notes :
| Library Type | Sample Type | Amount (Qubit) | Volume | Concentration | Purity (NANODROPTM/AGAROSE GEL) |
|
|---|---|---|---|---|---|---|
| Strongly Recommended | Required | |||||
| < 500 bp Insert Re Sequencing |
Genomic DNA | > 2 ug | > 1 ug | > 20 uL | > 50 ng/uL | No degradation, no contamination |
| Genomic DNA (PCR-free) | > 3 ug | > 1.5 ug | > 20 uL | > 50 ng/uL | No degradation, no contamination | |
| Metagenomics Library | Genomic DNA | > 2 ug | > 1 ug | > 30 uL | > 20 ng/uL | No degradation, no contamination |
| Amplicon | Genomic DNA | > 240 ng (ITS) > 120 ng (16S) |
> 20 uL | > 12 ng/uL (ITS) > 6 ng/uL (16S) |
No degradation, no contamination | |
| PCR Products* | > 400 ng | > 10 uL | > 20 ng/uL | Fragment size <450 bp no degradation, no contamination |
||
| PCR Products* | > 200 ng | > 10 uL | > 20 ng/uL | Fragment size < 450 bp no degradation, no contamination |
||
* One PCR product for one library
** Multiple PCR products for one library (at least 2 different PCR products)