Small RNA Sequencing

SAMPLE REQUIREMENTS

Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, NGB Diagnostics has extensive sample quality control procedures to ensure submitted samples conform to requirements for downstream processing.To guarantee the normal processing of your project, samples should meet the standards given below

Notes :

  • Samples not meeting these specifications should be designated as “at risk” by the customer, and will be subject to billing regardless of data quality. Please consult the Project Manager for further details.
  • Input quantity should be determined by Qubit® instead of by NanoDropTM, and the final quantity and concentration should conform to NGB Diagnostics specifications
Library Type Sample Type Amount (Qubit) Volume Concentration RNA INTEGRITY NUMBER(AGILENT 2100) Purity
( NANODROP ™ )
Strongly Recommended Required
Eukaryotic small RNA Sequencing Total RNA (Animal) > 6 ug > 3 ng > 20 uL > 50 ng/uL > 8, smooth base line No degradation,
no contamination
Total RNA
(Plant and Fungus)
> 6 ug > 3 ug 20 uL > 50 ng/uL > 7.5, smooth base line