SAMPLE REQUIREMENTS
Sample quality directly impacts sequencing quality and subsequent bioinformatics analysis. Therefore, NGB Diagnostics has extensive sample quality control procedures to ensure submitted samples conform to requirements for downstream processing.To guarantee the normal processing of your project, samples should meet the standards given below
Notes :
| Library Type | Sample Type | Amount (Qubit) | Volume | Concentration | Purity (NANODROPTM/AGAROSE GEL) | |
|---|---|---|---|---|---|---|
| Strongly Recommended | Required | |||||
| Human Whole Genome / Exome Sequencing / Target Region Capture |
Genomic DNA | > 2 ug | > 1 ug | > 20 uL | > 20 ng/uL | No degradation, no contamination |
| FFPE* | > 3 ug | > 1.5 ug | - | - | Fragments should be longer than 1500 bp | |
| Genomic DNA (PCR-free) |
> 3 ug | > 1.5 ug | > 20 uL | > 20 ng/uL | No degradation, no contamination | |
| DNA products of single-cell whole genome amplification |
> 2 ug | > 1 ug | > 20 uL | > 20 ng/uL | Fragments should be longer than 500 bp | |